non targeting sgrna controls scr Search Results


multiguide knockout kits (v2)  (Synthego Inc)
90
Synthego Inc multiguide knockout kits (v2)
Multiguide Knockout Kits (V2), supplied by Synthego Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/multiguide knockout kits (v2)/product/Synthego Inc
Average 90 stars, based on 1 article reviews
multiguide knockout kits (v2) - by Bioz Stars, 2026-04
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sgrnas targeting 606 ubiquitin e3 ligases, as well as 243 non-targeting control (ntc) sequences  (Twist Bioscience)
90
Twist Bioscience sgrnas targeting 606 ubiquitin e3 ligases, as well as 243 non-targeting control (ntc) sequences
A Schematic depicting the CRISPR screening strategy. RPE1‐Cas9i‐mt‐mKeima cells were transduced with a lentiviral CRISPR sgRNA library targeting <t>606</t> <t>E3</t> ligases. sgRNA‐expressing cells were selected for 7 days with puromycin (Puro) and Cas9 expression induced with doxycycline (Dox) for 9 days. Fourteen days post‐transduction, half the cells were treated with antimycin and oligomycin (AO; 1 and 10 μM respectively) for 24 h, then sorted alongside untreated cells (basal mitophagy) by FACS into “high” and “low mitophagy” populations based on mt‐mKeima fluorescence. Genomic DNA was extracted from sorted and unsorted reference cells, <t>sgRNAs</t> amplified by barcoded PCRs and samples analysed by next‐generation sequencing (NGS). B, C Volcano plot showing the average log 2 fold change and −Log 10 P ‐value of genes in low mitophagy versus unsorted cells in the AO‐induced and basal mitophagy screen for two independent biological replicates. Statistical thresholds of 2 and 3 standard deviations from the mean are indicated by dashed lines and colour coding. Indicated are high‐confidence (unbroken line) and lower‐confidence (dashed line) candidates shown in (D–F). D, E High‐confidence candidate list of positive (decreased) and negative (enhanced) regulators of Parkin‐independent‐induced mitophagy. Heatmap showing the Log 2 fold change of genes in low/high mitophagy versus unsorted cells in the induced and basal mitophagy screen for each of two independent biological replicates. Genes with P ‐values < 0.05 are indicated by an asterisk. F Venn diagram showing the overlap of genes listed in (D) and (E) that were identified in the basal and AO‐induced mitophagy screens as positive (green) and negative (magenta) regulators. Bold type indicates high‐confidence hits and regular type indicates lower‐confidence hits. Source data are available online for this figure.
Sgrnas Targeting 606 Ubiquitin E3 Ligases, As Well As 243 Non Targeting Control (Ntc) Sequences, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sgrnas targeting 606 ubiquitin e3 ligases, as well as 243 non-targeting control (ntc) sequences/product/Twist Bioscience
Average 90 stars, based on 1 article reviews
sgrnas targeting 606 ubiquitin e3 ligases, as well as 243 non-targeting control (ntc) sequences - by Bioz Stars, 2026-04
90/100 stars
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sgrnaexpressing vectors targeting met, shp2, ptp4a1 and non-targeting control  (Applied Biological Materials Inc)
90
Applied Biological Materials Inc sgrnaexpressing vectors targeting met, shp2, ptp4a1 and non-targeting control
A Schematic depicting the CRISPR screening strategy. RPE1‐Cas9i‐mt‐mKeima cells were transduced with a lentiviral CRISPR sgRNA library targeting <t>606</t> <t>E3</t> ligases. sgRNA‐expressing cells were selected for 7 days with puromycin (Puro) and Cas9 expression induced with doxycycline (Dox) for 9 days. Fourteen days post‐transduction, half the cells were treated with antimycin and oligomycin (AO; 1 and 10 μM respectively) for 24 h, then sorted alongside untreated cells (basal mitophagy) by FACS into “high” and “low mitophagy” populations based on mt‐mKeima fluorescence. Genomic DNA was extracted from sorted and unsorted reference cells, <t>sgRNAs</t> amplified by barcoded PCRs and samples analysed by next‐generation sequencing (NGS). B, C Volcano plot showing the average log 2 fold change and −Log 10 P ‐value of genes in low mitophagy versus unsorted cells in the AO‐induced and basal mitophagy screen for two independent biological replicates. Statistical thresholds of 2 and 3 standard deviations from the mean are indicated by dashed lines and colour coding. Indicated are high‐confidence (unbroken line) and lower‐confidence (dashed line) candidates shown in (D–F). D, E High‐confidence candidate list of positive (decreased) and negative (enhanced) regulators of Parkin‐independent‐induced mitophagy. Heatmap showing the Log 2 fold change of genes in low/high mitophagy versus unsorted cells in the induced and basal mitophagy screen for each of two independent biological replicates. Genes with P ‐values < 0.05 are indicated by an asterisk. F Venn diagram showing the overlap of genes listed in (D) and (E) that were identified in the basal and AO‐induced mitophagy screens as positive (green) and negative (magenta) regulators. Bold type indicates high‐confidence hits and regular type indicates lower‐confidence hits. Source data are available online for this figure.
Sgrnaexpressing Vectors Targeting Met, Shp2, Ptp4a1 And Non Targeting Control, supplied by Applied Biological Materials Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sgrnaexpressing vectors targeting met, shp2, ptp4a1 and non-targeting control/product/Applied Biological Materials Inc
Average 90 stars, based on 1 article reviews
sgrnaexpressing vectors targeting met, shp2, ptp4a1 and non-targeting control - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


A Schematic depicting the CRISPR screening strategy. RPE1‐Cas9i‐mt‐mKeima cells were transduced with a lentiviral CRISPR sgRNA library targeting 606 E3 ligases. sgRNA‐expressing cells were selected for 7 days with puromycin (Puro) and Cas9 expression induced with doxycycline (Dox) for 9 days. Fourteen days post‐transduction, half the cells were treated with antimycin and oligomycin (AO; 1 and 10 μM respectively) for 24 h, then sorted alongside untreated cells (basal mitophagy) by FACS into “high” and “low mitophagy” populations based on mt‐mKeima fluorescence. Genomic DNA was extracted from sorted and unsorted reference cells, sgRNAs amplified by barcoded PCRs and samples analysed by next‐generation sequencing (NGS). B, C Volcano plot showing the average log 2 fold change and −Log 10 P ‐value of genes in low mitophagy versus unsorted cells in the AO‐induced and basal mitophagy screen for two independent biological replicates. Statistical thresholds of 2 and 3 standard deviations from the mean are indicated by dashed lines and colour coding. Indicated are high‐confidence (unbroken line) and lower‐confidence (dashed line) candidates shown in (D–F). D, E High‐confidence candidate list of positive (decreased) and negative (enhanced) regulators of Parkin‐independent‐induced mitophagy. Heatmap showing the Log 2 fold change of genes in low/high mitophagy versus unsorted cells in the induced and basal mitophagy screen for each of two independent biological replicates. Genes with P ‐values < 0.05 are indicated by an asterisk. F Venn diagram showing the overlap of genes listed in (D) and (E) that were identified in the basal and AO‐induced mitophagy screens as positive (green) and negative (magenta) regulators. Bold type indicates high‐confidence hits and regular type indicates lower‐confidence hits. Source data are available online for this figure.

Journal: The EMBO Journal

Article Title: FBXL4 ubiquitin ligase deficiency promotes mitophagy by elevating NIX levels

doi: 10.15252/embj.2022112799

Figure Lengend Snippet: A Schematic depicting the CRISPR screening strategy. RPE1‐Cas9i‐mt‐mKeima cells were transduced with a lentiviral CRISPR sgRNA library targeting 606 E3 ligases. sgRNA‐expressing cells were selected for 7 days with puromycin (Puro) and Cas9 expression induced with doxycycline (Dox) for 9 days. Fourteen days post‐transduction, half the cells were treated with antimycin and oligomycin (AO; 1 and 10 μM respectively) for 24 h, then sorted alongside untreated cells (basal mitophagy) by FACS into “high” and “low mitophagy” populations based on mt‐mKeima fluorescence. Genomic DNA was extracted from sorted and unsorted reference cells, sgRNAs amplified by barcoded PCRs and samples analysed by next‐generation sequencing (NGS). B, C Volcano plot showing the average log 2 fold change and −Log 10 P ‐value of genes in low mitophagy versus unsorted cells in the AO‐induced and basal mitophagy screen for two independent biological replicates. Statistical thresholds of 2 and 3 standard deviations from the mean are indicated by dashed lines and colour coding. Indicated are high‐confidence (unbroken line) and lower‐confidence (dashed line) candidates shown in (D–F). D, E High‐confidence candidate list of positive (decreased) and negative (enhanced) regulators of Parkin‐independent‐induced mitophagy. Heatmap showing the Log 2 fold change of genes in low/high mitophagy versus unsorted cells in the induced and basal mitophagy screen for each of two independent biological replicates. Genes with P ‐values < 0.05 are indicated by an asterisk. F Venn diagram showing the overlap of genes listed in (D) and (E) that were identified in the basal and AO‐induced mitophagy screens as positive (green) and negative (magenta) regulators. Bold type indicates high‐confidence hits and regular type indicates lower‐confidence hits. Source data are available online for this figure.

Article Snippet: sgRNAs targeting 606 Ubiquitin E3 ligases, as well as 243 non‐targeting control (NTC) sequences, were designed by Azimuth2 (Doench et al , ) and the top four picks (on‐target scores > 0.6) were chosen per gene, extended by 5 and 3 prime 3Cs homology and obtained as a pool from Twist Bioscience (Dataset ).

Techniques: CRISPR, Transduction, Expressing, Fluorescence, Amplification, Next-Generation Sequencing